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And Beato, M. C19B3D. Nature 3Q1, 749. , and Beato, M. C1996). DNA, in press. , and Berlin, C M . C1965). J. Biol. Chem. 240T 322. , and Lituack, G. C19B6). In "Glucocorticoid Hormones. Mechanism of Action" CY. Sakamoto and F. ), p. 35. Japan Sei. Soc. Press/Springer-Uerlag. O. C19B5) J. Biol. Chem. 260. 1492. , and Feigelson, P. (1973). Proc. Natl. Acad. Sei. USA 70, 1218. , and Schütz, ß. (1984). Proc. Natl. Acad. Sei. USA ELL 134B. E. (1980). Cell £1, 773. , and Beato, fl. (1985). Mol. Cell.

Cell g, S99. , and Axel, R. C19B2). Cell £&, 6E3. , and Uorcel, A. C19B4). Cell 3Z, 21. , and Ulorcel, A. C13B5). Cell Ifi, 923. B. C19B6D. In "Glucocorticoid Hormones. Mechanism of Action". CY. Sakamoto and F. ), p. 3. Japan Sei. Soc. Press/ Springer-Uerlag. Scheidereit, C , and Beato, M. C19B4D. Proc. Natl. Acad. Sei. USAfll,3029. , and Beato, M. C19B3D. Nature 3Q1, 749. , and Beato, M. C1996). DNA, in press. , and Berlin, C M . C1965). J. Biol. Chem. 240T 322. , and Lituack, G. C19B6). In "Glucocorticoid Hormones.

3. The effects of phenylephrine (10 μΗ) and glucagon (10 nM) are shown more clearly in Figs. 4 and 5, respectively. Strikingly different Signalling Systems in Stimulus-Response Coupling 47 quantitative increases of Ins(l,4,5)P3 above background were observed for the three agonists despite the fact that they each increase the cytosolic free Ca2+, as first reported by Charest et al. (1983). With vasopressin and phenylephrine at maximum effective concentrations, the peak Ca2 is reached after 5-10 s, while with glucagon a slower increase peaking after 30-40 s is obtained (see Fig.

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